Molecular Basis of Inheritance

AGGTATCGCAT is a sequence from the coding strand of a gene. What will be the corresponding sequence of the transcribed mRNA?

1. AGGUAUCGCAU
2. UGGTUTCGCAT
3. ACCUAUGCGAU
4. UCCAUAGCGUA

All of the following are part of an operon except

1. an operator
2. structural genes
3. an enhancer
4. a promoter

The final proof for DNA as the genetic material came from the experiments of

1. Griffith
2. Hargobind Khorana
3. Avery, MacLeod and McCarty
4. Hershey and Chase

If there are 999 bases in an RNA that codes for a protein with 333 amino acids, and the base at position 901 is deleted such that the length of the RNA becomes 998 bases, how many codons will be altered ?

1. 1
2. 11
3. 333
4. 33

During DNA replication, Okazaki fragments are used to elongate

1. The leading strand away from replication fork
2. The lagging strand towards replication fork
3. The lagging strand away from the replication fork
4. The leading strand towards replication fork

Which of the following RNAs should be most abundant in animal cell ?

1. m-RNA
2. mi-RNA
3. r-RNA
4. t-RNA

Spliceosomes are not found in cells of :

1. Bacteria
2. Plants
3. Animals
4. Fungi

The association of histone H1 with a nucleosome indicates :

1. DNA replication is occurring
2. Transcription is occurring
3. The DNA is condensed into a Chromatin Fibre
4. The DNA double helix is exposed

Which one of the following is the starter codon ?

1. AUG
2. UAG
3. UAA
4. UGA

Which of the following is required as inducer(s) for the expression of Lac operon?

1. lactose
2. lactose and galactose
3. glucose
4. galactose

A complex of ribosomes attached to a single strand of RNA is known as :

1. Polypeptide
2. Okazaki fragment
3. Polysome
4. Polymer

Which of the following is not required for any of the techniques of DNA fingerprinting available at present?

1. DNA – DNA hybridization
2. Polymerase chain reaction
3. Zinc finger analysis
4. Restriction enzymes

Taylor conducted the experiments to prove semiconservative mode of chromosome replication on

1. Vicia faba
2. Drosophila melanogaster
3. Vinca rosea
4. E. coli

The equivalent of a structural gene is -

1. cistron
2. recon
3. muton
4. operon

Which of the following rRNAs acts as structural RNA as well as ribozyme in bacteria ?

1. 5.8 S rRNA
2. 23 S rRNA
3. 18 S rRNA
4. 5 S rRNA

A molecule that can act as a genetic material must fulfill the traits given below, except

1. it should be able to express itself in the form of ‘Mendelian characters’
2. it should be unstable structurally and chemically
3. it should provide the scope for slow changes that are required for evolution
4. it should be able to generate its replica

DNA-dependent RNA polymerase catalyzes transcription on one strand of the DNA which is called the

1. coding strand
2. antistrand
3. template strand
4. alpha strand

Which one of the following is not applicable to RNA?

1. Complementary base pairing
2. 5' phosphoryl and 3' hydroxyl ends
3. Heterocyclic nitrogenous bases
4. Chargaff's rule

Identify the correct order of organisation of genetic material from largest to smallest :

1. Chromosome, genome, nucleotide, gene
2. Genome, chromosome, nucleotide, gene
3. Genome, chromosome, gene, nucleotide
4. Chromosome, gene, genome, nucleotide

Satellite DNA is important because it :

1. Shows high degree of polymorphism in population and also the same degree of polymorphism in an individual, which is heritable from parents to children.
2. does not code for proteins and is same in all members of the population.
3. codes for enzymes needed for DNA replication.
4. codes for proteins needed in cell cycle.

Gene regulation governing lactose operon of E. coli that involves the lac I gene product is

1. negative and inducible because repressor protein prevents transcription.
2. positive and inducible because it can be induced by lactose
3. negative and repressible because repressor protein prevents transcription
4. feedback inhibition because excess of -galactosidase can switch off transcription

In sea urchin DNA, which is double stranded, 17% of the bases were shown to be cytosine. The percentages of the other three bases expected to be present in this DNA are

1. G 17%, A 16.5%, T 32.5%
2. G 17%, A 33%, T 33%
3. G 8.5%, A 50%, T 24.5%
4. G 34%, A 24.5%, T 24.5%

Transformation was discovered by :

1. Griffith
2. Watson and Crick
3. Hershey and Chase
4. Meselson and Stahl

Select the correct option ;

1. Direction of RNA synthesis 3'–5', Direction of reading of the template DNA strand 5'–3'
2. Direction of RNA synthesis 5'–3', Direction of reading of the template DNA strand 5'–3'
3. Direction of RNA synthesis 5'–3', Direction of reading of the template DNA strand 3'–5'
4. Direction of RNA synthesis 3'–5', Direction of reading of the template DNA strand 3'–5'

An analysis of chromosomal DNA using the Southern hybridization technique does not use:

1. Blotting
2. PCR
3. Electrophoresis
4. Autoradiography

Commonly used vectors for human genome sequencing are :

1. BAC and YAC
2. T/A Cloning Vectors
3. T-DNA
4. Expression Vectors

The diagram shows an important concept in the genetic implication of DNA. Fill in the blanks A to C. [figure]

1. A-translation B-extension C-Rosalind Franklin
2. A-translation B-transcription C-Erwin Chargaff
3. A-transcription B-translation C-Francis Crick
4. A-transcription B-replication C-James Watson

Which enzyme will be produced in a cell in which there is a non-sense mutation in the lac Y gene ?

1. Transacetylase
2. Lactose permease and transacetylase
3. Lactose permease
4. -galactosidase

[figure] The figure gives an important concept in the genetic implication of DNA. Fill the blanks A, B and C.

1. A - Erwin Chargaff B - translation C - replication
2. A - Maurice Wilkins B - transcription C - translation
3. A - Francis Crick B - translation C - transcription
4. A - James Watson B - replication C - extension

Which of the following is not a property of the genetic code ?

1. Ambiguous
2. Universal
3. Degeneracy
4. Non-overlapping